May 15, 2024
Description
In most molecular biology labs the western blot membranes are incubated in flat trays, flat plastic bags or tubings. This results mostly in poor distribution of the antibody or staining solution and therefore uneven spreading of the antibody. This tray has some elevators on the ground that the antibody solution can incorporate from both sides of the membrane. The round corners leads to a better flow of the solution if the tray is placed on a rocker (anyway the best results of such a stain takes place on a rocker and not an oribital shaker). The size of the elevators was tested in several experiments and found to perform as the best.
The surface contact from the elevators to the membrane is also minimized for nearly full coverage of solution from below. The fluidic dynamics will also alow the membrane to slide a couple of millimeters if placed on a rocker.
Furthermore the constant intermixing of the staining solution leads also to a more homogenous distribution.
If printed with black PETG (alternatively ASA or ABS) it also provides protection for light-sensitive fluorescence labelled antibodies.
Sure the staining with other solutions like Coomassie is also possible.
The tray was made for one mini size membrane - like for Bio-Rad Tetracell gel size.
License:
Creative Commons — Attribution — Noncommercial